Journal: Bioengineering

Article Title: Development of an Eye Irritation Test Method Using an In-House Fabrication of a Reconstructed Human Cornea-like Epithelium Model for Eye Hazard Identification

doi: 10.3390/bioengineering11040302

Figure Lengend Snippet: ( a ) Monitoring the recovery of the iHCE-NY1 model using histological cross-sections. HE-stained tissue specimens of the iHCE-NY1 model’s incubation for 1- (post-incubation period (PIP) 1), 7-(PIP 7), 14-(PIP 14), and 21-days (PIP 21) post exposure to liquid chemicals are shown. At PIP 1, cells with enriched nuclei (yellow arrowheads) were observed, while viable cells (blue arrowheads) were observed from PIP 7. As the culture days increased to PIP 14 and PIP 21, more viable cells were observed. The gray dotted line indicates the position of the bottom of the iHCE-NY1 model. #6: γ-butyrolactone, #16: 2-methyl-1-pentanol, all bar indicates 50 µm. ( b ) Detection of respirating cells in the iHCE-NY1 model. Cells in S phase of the cell cycle that took up BrdU had cell nuclei revealed with red fluorescence. At post-incubation period (PIP) 1-day, few cells were in the S phase. From PIP 7-day onward, BrdU-positive S-phase cells were detected in all samples. #6: γ-butyrolactone, #16: 2-methyl-1-pentanol, all bar indicates 50 µm.

Article Snippet: Fixed and paraffin sections were prepared, and BrdU was detected using a sheep polyclonal anti-BrdU antibody (1:100, Capralogics, Hardwick, MA, USA) and an anti-sheep IgG antibody labeled with Alexa Fluor 594 (1:1000, Thermo Fisher Scientific Inc., Waltham, MA, USA).

Techniques: Staining, Incubation, Fluorescence

Journal: Stem Cells International

Article Title: Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway

doi: 10.1155/2017/5841814

Figure Lengend Snippet: Electroacupuncture (EA) treatment enhanced TBI-induced neurogenesis in the hippocampus after traumatic brain injury (TBI). (a) Coronal section of hippocampal dentate gyrus (DG), stained with BrdU (green fluorescence)/NeuN (red fluorescence). MOL, GCL, and SGZ referred, respectively, to molecular layer, granular cell layer, and subgranular zone in DG. The white pane representing one visual field under confocal laser scanning microscope was shown in (b). (b) Representative immunofluorescence (IF) microphotographs of SGZ in the sham, TBI, TBI + EA, and sham + EA groups ( n = 9 in each group) at 21, 28, and 35 days postinjury. The newly generated neurons were double labeled with BrdU/NeuN and merged into yellow. (c) Quantitation analysis revealed that, compared with the sham group, the number of BrdU/NeuN-positive cells was notably increased in the TBI group and EA treatment induced much more double-positive cells in the TBI + EA group than in the TBI and sham + EA groups at the three examined time points. Scale bar: 50 μ m. ∗ P < 0.05 versus the TBI group.

Article Snippet: Briefly, brain sections were incubated for 12 hours at 4°C with primary antibodies: anti-BrdU sheep polyclonal antibody (1 : 200, GeneTex, GTX21893, Irvine, CA, USA) and anti-NeuN rabbit monoclonal antibody (1 : 100, CST, 24307, Beverly, MA, USA).

Techniques: Staining, Fluorescence, Laser-Scanning Microscopy, Immunofluorescence, Generated, Labeling, Quantitation Assay

Journal: Stem Cells International

Article Title: Electroacupuncture Improved Hippocampal Neurogenesis following Traumatic Brain Injury in Mice through Inhibition of TLR4 Signaling Pathway

doi: 10.1155/2017/5841814

Figure Lengend Snippet: Lipopolysaccharide (LPS) was administrated in lateral ventricle of the brain to activate TLR4 signaling pathway. TLR4 activation attenuated the EA-induced neurogenesis in SGZ of the hippocampus after TBI. (a) Representative BrdU/NeuN double-labeled microphotographs of SGZ in TBI + EA, TBI + EA + LPS, and TBI + EA + Veh groups ( n = 9 in each group) at 21, 28, and 35 days posttrauma. (b) Statistical data showed that, compared with TBI + EA group, BrdU/NeuN positive cells were significantly decreased in TBI + EA + LPS group and maintained at the same level in TBI + EA + Veh group at the three examined time points. Scale bar: 50 μ m. † P < 0.05 versus the TBI + EA + LPS group.

Article Snippet: Briefly, brain sections were incubated for 12 hours at 4°C with primary antibodies: anti-BrdU sheep polyclonal antibody (1 : 200, GeneTex, GTX21893, Irvine, CA, USA) and anti-NeuN rabbit monoclonal antibody (1 : 100, CST, 24307, Beverly, MA, USA).

Techniques: Activation Assay, Labeling